mouse anti human a β 82e1  (Bio-Rad)

Journal: International Journal of Molecular Sciences

Article Title: APP Knock-In Mice Produce E22P-Aβ Exhibiting an Alzheimer’s Disease-like Phenotype with Dysregulation of Hypoxia-Inducible Factor Expression

doi: 10.3390/ijms232113259

Figure Lengend Snippet: Alzheimer’s disease (AD)-related histological changes in the brain of APP NL-P-F/NL-P-F mice. ( A ) The Aβ plaque deposition was detected by 82E1 in the hippocampal CA1, DG, and cortex of APP NL-P-F/NL-P-F mice from three to twelve months. The scale bar indicates 1 mm in representative coronal sections and 100 μm in each region). ( B ) The Aβ plaque area in the hippocampal CA1, DG, and the cortex of APP NL-P-F/NL-P-F mice from three to twelve months. WT ( n = 6) and NLPF ( n = 7–9). ( C , D ) Representative ( C ) fluorescent and ( D ) confocal images of tau phosphorylation were detected by PHF-1 (Ser396/Ser404) in the hippocampal CA1, CA3, and DG after twelve months. The scale bar indicates ( C ) 1 mm (50 μm in enlarged images) and ( D ) 50 μm (25 μm in enlarged images of CA1 area). ( E ) After twelve months, the phosphorylated tau-positive area (% of total area) in the hippocampal CA1, CA3, and DG. WT and NLPF ( n = 6). ( F , G ) After twelve months, representative ( F ) fluorescent and ( G ) confocal images of NeuN in the hippocampal CA1 and CA3. The scale bar indicates ( F ) 250 μm (50 μm in enlarged images) and ( G ) 50 μm. ( H ) After twelve months, the number of NeuN-positive neurons in the hippocampal CA1 and CA3. WT ( n = 17 slices/6 mice), NLPF ( n = 13 slices/6 mice). The values indicate the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with age-matched WT.

Article Snippet: Mouse Anti-Human Amyloid β antibody (82E1) , 1:1000 , Immuno-Biological Laboratories, Gunma, Japan , 10323.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: APP Knock-In Mice Produce E22P-Aβ Exhibiting an Alzheimer’s Disease-like Phenotype with Dysregulation of Hypoxia-Inducible Factor Expression

doi: 10.3390/ijms232113259

Figure Lengend Snippet: Primary antibodies used for immunohistochemistry (IHC).

Article Snippet: Mouse Anti-Human Amyloid β antibody (82E1) , 1:1000 , Immuno-Biological Laboratories, Gunma, Japan , 10323.

Techniques: Immunohistochemistry

Journal: STAR Protocols

Article Title: Phenotypic analysis of a transgenic Drosophila model of Alzheimer’s amyloid-β toxicity

doi: 10.1016/j.xpro.2021.100501

Figure Lengend Snippet:

Article Snippet: Amyloid β (N) (82E1) Anti-Human Mouse IgG MoAb antibody , Immuno-Biological Laboratories , Cat# 10323; RRID: AB_10707424.

Techniques: Recombinant, Electron Microscopy, Protease Inhibitor, Western Blot, Software

Journal: Cell systems

Article Title: Pulse-chase proteomics of the App Knock-In mouse models of Alzheimer’s disease reveals synaptic dysfunction originates in presynaptic terminals

doi: 10.1016/j.cels.2020.11.007

Figure Lengend Snippet: (A) Aβ42 levels in cortical homogenates (GuHCl soluble) from AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 2, 4, 6, 8, 10, and 12 months of age as measured by sandwich ELISA with analytical sensitivity range of 15.6 – 1,000 pg / mL. (B) Aβ42 levels in cortical homogenates (GuHCl soluble) from AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 4, 6, and 8 months of age as measured by an ultrasensitive sandwich ELISA with analytical sensitivity range of 1.56 – 100 pg / mL. (C) Oligomeric Aβ42 levels in TBST soluble cortical extracts from AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 4, 6, and 8 months of age as measured by a sandwich ELISA using the oligomeric preferential MOAB-2 antibody. (D) Amyloid pathology in App KI cortex and hippocampus. Representative thioflavin S stained sagittal brain sections from 6- and 12-month-old AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice. Scale bar = 500 μm. (E) Quantification of amyloid plaque area from panel (D). Data represents average thioflavin S positive stained area relative to the total cortical or hippocampal area per section. (F) Amyloid pathology in App KI cerebellum. Representative brain sections from 6- and 12-month-old AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice stained with thioflavin S. Scale bar = 500 μm. (G) Quantification of amyloid plaque area from panel (F) Data represents average thioflavin S positive stained area relative to the total cerebellar area per section. (H) Dot blot analysis of Aβ (82E1), amyloid fibrils (LOC), and actin using aggregated protein fractions from cortical extracts of AppNL/NL, AppNL-F/NL-F, and AppNL-G-F/NL-G-F mice at 6 months of age. (I) Quantification of Aβ (82E1) and amyloid fibrils (LOC) levels from panel (H). (A) N = 3 – 8 mice of mixed gender, (B-C) N = 4 mice of mixed gender, (E, G, I) N = 3 mice of mixed gender, per group. Each datapoint was an average of 2 – 3 30 μm sections per mouse. All data represent mean ± SD, analyzed by one-way ANOVA followed by Fisher’s LSD. Circles represent individual biological replicates. * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001.

Article Snippet: The following primary antibodies were used for Western and dot blots: Anti-Human Amyloid-beta (N) (82E1) (also detects β-CTF) mouse monoclonal at 1:1,000 (Immuno-Biological Laboratories Cat# 10323, RRID: AB_10707424); Anti-Amyloid beta precursor protein [Y188] rabbit monoclonal at 1:1,000 (Abcam Cat# ab32136, RRID: AB_2289606); Anti-AP180 (Snap91) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 155 003, RRID: AB_887691); Anti-Gapdh (0411) mouse monoclonal at 1:2,000 (Santa Cruz Biotechnology Cat# sc-47724, RRID: AB_627678); Anti-Pip5k1c rabbit polyclonal at 1:500 (Novus Cat# NBP1–82986, RRID: AB_11029240); Anti-Snap25 rabbit polyclonal at 1:500 (Synaptic Systems Cat# 111 002, RRID: AB_887790); Anti-Synaptobrevin 2 (Vamp2) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 104 202, RRID: AB_887810); Anti-Synaptophysin mouse monoclonal at 1:1,1000 (Sigma-Aldrich Cat# S5768, RRID: AB_477523); Anti-Synaptotagmin 1/2 cytoplasmic tail rabbit polyclonal at 1:500 (Synaptic Systems Cat# 105 003AF, RRID: AB_2744565); Anti-Syntaxin 1B rabbit polyclonal at 1:500 (Synaptic Systems Cat# 110 402, RRID: AB_887901); Anti-Vamp1 rabbit polyclonal at 1:500 (Abcam Cat# ab41324, RRID: AB_1281203); Anti-Ubiquitin P4D1 mouse monoclonal at 1:1,000 (Santa Cruz Biotechnology Cat# sc-8017, RRID: AB_628423); Anti-VCP mouse monoclonal at 1:2,000 (Abcam Cat# ab11433, RRID: AB_298039).

Techniques: Sandwich ELISA, Staining, Dot Blot

Journal: Cell systems

Article Title: Pulse-chase proteomics of the App Knock-In mouse models of Alzheimer’s disease reveals synaptic dysfunction originates in presynaptic terminals

doi: 10.1016/j.cels.2020.11.007

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following primary antibodies were used for Western and dot blots: Anti-Human Amyloid-beta (N) (82E1) (also detects β-CTF) mouse monoclonal at 1:1,000 (Immuno-Biological Laboratories Cat# 10323, RRID: AB_10707424); Anti-Amyloid beta precursor protein [Y188] rabbit monoclonal at 1:1,000 (Abcam Cat# ab32136, RRID: AB_2289606); Anti-AP180 (Snap91) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 155 003, RRID: AB_887691); Anti-Gapdh (0411) mouse monoclonal at 1:2,000 (Santa Cruz Biotechnology Cat# sc-47724, RRID: AB_627678); Anti-Pip5k1c rabbit polyclonal at 1:500 (Novus Cat# NBP1–82986, RRID: AB_11029240); Anti-Snap25 rabbit polyclonal at 1:500 (Synaptic Systems Cat# 111 002, RRID: AB_887790); Anti-Synaptobrevin 2 (Vamp2) rabbit polyclonal at 1:500 (Synaptic Systems Cat# 104 202, RRID: AB_887810); Anti-Synaptophysin mouse monoclonal at 1:1,1000 (Sigma-Aldrich Cat# S5768, RRID: AB_477523); Anti-Synaptotagmin 1/2 cytoplasmic tail rabbit polyclonal at 1:500 (Synaptic Systems Cat# 105 003AF, RRID: AB_2744565); Anti-Syntaxin 1B rabbit polyclonal at 1:500 (Synaptic Systems Cat# 110 402, RRID: AB_887901); Anti-Vamp1 rabbit polyclonal at 1:500 (Abcam Cat# ab41324, RRID: AB_1281203); Anti-Ubiquitin P4D1 mouse monoclonal at 1:1,000 (Santa Cruz Biotechnology Cat# sc-8017, RRID: AB_628423); Anti-VCP mouse monoclonal at 1:2,000 (Abcam Cat# ab11433, RRID: AB_298039).

Techniques: Recombinant, Sequencing, Modification, Blocking Assay, Bicinchoninic Acid Protein Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Software, Microscopy, Mass Spectrometry